Fig. 8

Enhanced activity of PKCδ mediates the increase in GluN2B phosphorylation at Ser1303, the reduced association to membrane of CaMKII, and the reduced phosphorylation at Ser831 and membrane expression of GluA1 in hyperammonemic rats. Rottlerin, an inhibitor for PKCδ, was added to hippocampal slices. Phosphorylation of GluN2B subunit at Ser1303 (a) and GluA1 at Ser831 (c) and membrane expression of CaMKII (b) and GluA1 (d) were analyzed as described in the “Methods” section. Values are expressed as percentage of basal levels in control rats and are the mean ± SEM of 15, 10, 29, and 21 rats per group in a, b, c, and d respectively. Data were analyzed by two-way ANOVA. In a, F (1, 55) = 0.5387 for effect of HA, p = 0.4661; F (1, 55) = 5.649 for effect of rottlerin, p = 0.0210; and F (1, 55) = 8.403 for interaction, p = 0.0054. In b, F (1, 36) = 1.445 for effect of HA, p = 0.2372; F (1, 36) = 9.889 for effect of rottlerin, p = 0.0033; and F (1, 36) = 13.77 for interaction, p = 0.0007. In c, F (1, 113) = 1.353 for effect of HA, p = 0.2471; F (1, 113) = 11.81 for effect of rottlerin, p = 0.0008; and F (1, 113) = 16.36 for interaction, p < 0.0001. In d, F (1, 80) = 2.696 for effect of HA, p = 0.1045; F (1, 80) = 6.059 for effect of rottlerin, p = 0.0160; and F (1, 80) = 9.431 for interaction, p = 0.0029. Values significantly different from control rats are indicated by asterisk and from hyperammonemic rats are indicated by “a”. Bonferroni post-test: ^*p < 0.05, ^**p < 0.01, ^***p < 0.01 ^aap < 0.01, ^aaap < 0.001