Fig. 2

SC-NSC activation and differentiation upon SCI. a Representative confocal images showing the central canal of healthy Nestin ^floxGFP^flox-TK mice labeled for GFP and BrdU. b, c Segments proximal (b) and distal (c) to the lesion epicenter from a Nestin ^floxGFP^flox-TK mouse sampled 14 days after SCI. d–f Quantifications of GFP⁺BrdU⁺ cells (means ± S.E.M) within the ependymal layer of the central canal in Nestin ^floxGFP^flox-TK mice sacrificed 3, 14, and 21 days after SCI (n = 3 mice/time point). g–j Nestin^CreERT2Rosa 26^YFP mice were injected with TAM for 5 days and, following a washout of further 5 days, subjected to injury. Confocal images of OLIG2/YFP (arrows indicate double positive cells), GFAP⁺YFP⁺, and NeuN⁺YFP⁺ are provide in g–i. Quantifications (means ± S.E.M.) are plotted on j, (n = 3 for each group). Two-way ANOVA, followed by Bonferroni’s multiple comparison test has been used to analyze data of j. Scale bar 30 μm