Fig. 5
Downregulation of neurotrophic factors in GCV-NestinTK mice. Real-time PCR for Igf1 (a), Lif (b), CNTF (c), and Vegfa (d) performed on total RNA extracts derived from T11–T13 spinal cord segments. Explants were collected at days 7 and 31. Values indicate the mean fold changes ± S.D. (n = 3–6 for each group) calculated on the expression levels measured in healthy mice (black bars). Comparisons were done using the t Student test: Igf1:***p = 0.0001, *p = 0.03, and p = 0.006; Cntf, p = 0.032; Vegfa, p = 0.008