Fig. 2

T. gondii infection induced Aβ immunoreactivity in the mouse brain. a Control brains and brains from mice orally infected with 10 ME49 cysts for 15, 30, 60, and 90 days were collected after perfusion with ice cold PBS. Sections were fixed and stained with a human anti-beta amyloid antibody, 6E10 (green) and an anti-T. gondii antibody BAG1 (red) and counterstained with DAPI (blue). n = 5 mice per group. Control sections were stained with anti-T. gondii antibody. b Quantification of 6E10-positive signal from 15 cortical regions from brains of mice at 30, 60, and 90 days post infection, n = 5 mice group. c Quantification of 6E10-positive signal from 10 hippocampal regions at 30, 60, and 90 days post infection. n = 5 mice per group. d Cryo-brain sections were stained with Thioflavin S (green) and anti-T. gondii (red) antibodies and counterstained with DAPI (blue). Representative images from T. gondii colocalized with 6E10 (top), as well as T gondii located distally from 6E10⁺ areas (bottom) are shown; n = 2–3 mice per group. Data are shown as mean ± SEM. *p < 0.05, **p < 0.01