Fig. 2

Effect of Ang II on hippocampal gliosis. CD68 mRNA (a) and GFAP mRNA (b) gene expression were analyzed by qRT-PCR in microdissected hippocampi after 1 week systemic perfusion of Ang II 200 or 1000 ng/kg/min or 0.9% saline (CTL). In each experiment, a treated-to-control ratio was calculated (*p < 0.05, Ang II 1000 versus control, by one-way ANOVA with Tukey post-test, n = 4–5). c–e Relative fluorescence intensity units (RFU) of CD68 (c), Iba-1 (d), and GFAP (e) in the hippocampus after 1 week systemic perfusion of Ang II 200 or 1000 ng/kg/min or 0.9% saline (CTL). In each experiment, a treated-to-control ratio was calculated (**p < 0.01, Ang II 1000 versus control, by one-way ANOVA with Tukey post-test, n = 4–6). Representative micrographs of CD68, Iba-1, and GFAP immunostainings in DG area of hippocampus are shown below each graph (scale 50 μm)