Fig. 6
RNS60 reduces NMJ denervation and promotes macrophage recruitment in TAM of C57BL/6-SOD1G93A mice. a–c Representative confocal images of co-localization of synaptophysin (SYN, green) with bungarotoxin (BTX, red) in TAM of NTG mice and C57BL/6-SOD1G93A mice treated with NS or RNS60 (scale bar 50 μm). d Denervation of NMJ was higher in TAM of transgenic mice treated with NS as compared to NTG (61.8 ± 2.8% vs. 22.3 ± 2.75). Treatment with RNS60 slightly but significantly reduced this percentage (52.4 ± 2.5%). Data are expressed as mean ± SEM, (n = 5 animals per group). e–g Representative images of TAM of NTG, SOD1G93A NS and SOD1G93A RNS60 stained for CD68 (green), scale bar: 50 μm. Inserts show CD68+ cell infiltration at the level of NMJ labeled with bungarotoxin (red) at higher magnification, scale bar: 10 μm. h The quantification of immunoreactivity showed an increase of CD68+ cells in transgenic mice treated with NS compared to NTG. The treatment with RNS60 exacerbated the recruitment of macrophages. Data are expressed as mean ± SEM, (n = 5 animals per group). All data were statistically analysized using one-way ANOVA followed by post hoc Fisher’s LSD, *p < 0.05, **p < 0.01, ***p < 0.001