Fig. 9
From: Nitrosative damage during retrovirus infection-induced neuropathic pain
NO-induced damage within the LSC and DRG of LP-BM5 infected PD-1 KO animals. a Western blot of LSC tissue lysates from uninfected (UI) and LP-BM5-infected (Inf) WT (UI, lanes 1 and 2; Inf, lanes 3 and 4) and PD-1 KO (UI, lanes 5 and 6; Inf, lanes 7 and 8) animals probed with anti-3-nitrotyrosine antibodies. Each lane displays tissue extract from one individual animal at 10 weeks p.i. The band labeled a identifies a high molecular weight (MW) protein band, while the b band points out a lower MW protein, both of which are nitrosylated. The signal intensity of both a and b protein bands observed in the lysates of uninfected and LP-BM5-infected WT and PD-1 KO animals was measured using densitometry and plotted alongside. *p < 0.05, **p < 0.01 (b) western blot of lysates obtained from uninfected (UI) and LP-BM5-infected (IF) WT (UI, lane 1 and IF, lane 2) and PD-1 KO (UI, lane 3 and IF, lane 4) animals’ DRG (L3-L5) probed with anti-3-nitrotyrosine antibodies. Each lane displays tissue extract pooled from 3 animals at 10 weeks p.i. The band labeled a identifies a high MW protein band, and b represents the lower MW protein band that are nitrosylated. The signal intensity of both a and b protein bands observed was measured and plotted alongside. M = protein molecular weight marker ranging from size 15 to 250 kD. c iNOS expression was measured by real-time RT-PCR in the LSC (L3-L5) of LP-BM5-infected WT and PD-1 KO animals at 10 weeks p.i. (n = 4–6). d iNOS mRNA expression in DRG tissues at 10 weeks p.i. via real-time RT-PCR (n = 4–6). Results are presented as box plots of pooled data from individual animals