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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Early alpha-lipoic acid therapy protects from degeneration of the inner retinal layers and vision loss in an experimental autoimmune encephalomyelitis-optic neuritis model

Fig. 1

Alpha-lipoic acid as well as its enantiomers and DHLA show protective effects in a model of oxidative glutamate toxicity. Five thousand HT22 cells were seeded into 96-well plates and treated with either vehicle or different concentrations of LA, DHLA, (S)-LA, or (R)-LA, followed by glutamate 24 h later. Graphs represent curve fits ± SEM for 0.625, 1.25, and 2.5 mM (a) and 5 or 10 mM (b) glutamate (glu) of five independent experiments, each performed in triplicates with corresponding EC50 values. Significant differences between LA and DHLA, (R)-LA, or (S)-LA treatments are indicated by asterisks (*p < 0.05, area under the curve compared by ANOVA with Dunnett’s post hoc test). Structural formulas of the used compounds (c). For GSH, cells were treated with 25 μM LA for indicated time points and glutamate for additional 8 h before harvesting for protein extraction and enzymatic assay (d) (***p < 0.001, compared by two-way ANOVA with Dunnett’s post hoc test to vehicle treatment)

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Journal of Neuroinflammation

ISSN: 1742-2094