Fig. 4

CX₃CR₁ deficiency increases CCR₂ expression in vivo. a Representative blot for CCL₂ (15 kDa) in CX₃CR₁^+/GFP and CX₃CR₁^GFP/GFP sciatic nerves. b Quantification of CCL₂ band density normalised to α-tubulin (mean ± SEM, n = 3). Basal expression of CCL₂ in sciatic nerve homogenates is significantly higher in CX₃CR₁-deficient mice relative to saline-treated CX₃CR₁ heterozygous. VCR significantly increases CCL₂ in both genotypes. *p < 0.05, ***p < 0.005, one-way ANOVA, Tukey’s test. c Representative images of GFP (macrophages) and CCL₂ (red) in sciatic nerve transverse sections at day 11. Scale bar = 50 μm. d Quantification of the percentage of GFP+ profiles that are also CCL₂+. Two cycles of VCR significantly increases the percentage of GFP+/CCL₂+ macrophages in both genotypes (mean ± SEM, n = 4 mice per group). ***p < 0.005, one-way ANOVA, post hoc Tukey’s test. e Representative dot blots for peritoneal lavages. Numbers in the gates refer to percentage of positive cells. f Bar chart representing number of F4/80⁻Ly6C⁺CCR2⁺ events (mean ± SEM, n = 3). *p < 0.05, **p < 0.01 and ***p < 0.005, one-way ANOVA, Tukey’s test