Fig. 6

Celastrol treatment protects against OGD-induced neuronal apoptosis and inflammatory factor expression. Neuron-microglia co-cultures exposed to OGD conditions for 3 h were treated with celastrol (1 μM) or PBS (control) for 24 h. a Neuronal apoptosis was assessed with flow cytometry using annexin V-FITC staining, and b the relative apoptosis ratio was analyzed. The data are presented as mean ± SD. ^**P < 0.01, ^***P < 0.001 vs. control group. ^###P < 0.001 vs. OGD group. c–g ELISA results showing the expression of IL-33 (c), IL-1β (d), IL-6 (e), TNF-α (f), and IL-10 (g) in cell supernatant. The data are presented as mean ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. control group. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. OGD group. Each experiment repeated 6 times