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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Roles of astrocytic connexin-43, hemichannels, and gap junctions in oxygen-glucose deprivation/reperfusion injury induced neuroinflammation and the possible regulatory mechanisms of salvianolic acid B and carbenoxolone

Fig. 3

Evaluation of astrocytic GJIC permeability and hemichannel activity after OGD/R injury with SalB or CBX. a For GJIC detection, we measured calcein-AM transfer between “donor cells” and “acceptor cells” with flow cytometry. Shown here is a representative flow cytometry plot of transfer after donor astrocytes were labeled with calcein-AM and cocultured with acceptor astrocytes for 4 h. Grouped dye transfer data are shown in b. OGD/R injury decreased the degree of astrocytic coupling, but SalB reversed this effect. CBX further inhibited cellular coupling. c Representative images depicting ethidium uptake via hemichannels in the four groups. d OGD/R injury increased astrocytic ethidium uptake, but SalB and CBX achieved near-significant attenuation of this effect. e The supernatant ATP concentration was strongly elevated in the OGD/R group astrocytes, but SalB and CBX reversed this effect. We evaluated the statistical significance with ANOVA and Duncan’s multiple comparisons test. *p < 0.05, **p < 0.01, and ***p < 0.001. Scale bar = 50 μm

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Journal of Neuroinflammation

ISSN: 1742-2094