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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Roles of astrocytic connexin-43, hemichannels, and gap junctions in oxygen-glucose deprivation/reperfusion injury induced neuroinflammation and the possible regulatory mechanisms of salvianolic acid B and carbenoxolone

Fig. 6

Evaluation of impact of MCM on astrocytic GJIC permeability and hemichannel activity after OGD/R injury. a For GJIC detection, we measured calcein-AM transfer between “donor cells” and “acceptor cells” with flow cytometry. Shown here is a representative flow cytometry plot of transfer after donor astrocytes were labeled with calcein-AM and co-cultured with acceptor astrocytes for 4 h. Grouped dye transfer data are shown in b. OGD/R-MCM decreased the degree of astrocytic coupling, but OGD/R-SalB-MCM increased astrocytic intercellular dye transfer. c Representative images depicting ethidium uptake via hemichannels in the four groups. d OGD/R-MCM increased astrocytic ethidium uptake, but this effect was reversed in OGD/R-SalB-MCM-treated astrocytes. e OGD/R-MCM increased ATP concentrations in the astrocytic supernatant, but this effect was reversed by OGD/R-SalB-MCM. We evaluated the statistical significance with ANOVA and Duncan’s multiple comparisons test. *p < 0.05, **p < 0.01, and ***p < 0.001. Scale bar = 50 μm

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Journal of Neuroinflammation

ISSN: 1742-2094