Table 1 List of antibodies used in this study
Antibody | Source | Usage | Dilution factor |
|---|---|---|---|
Actin | Sigma (Rabbit, A2066) | WB | 1:300 |
Actin | Chemicon (Mouse, MAB1501R) | WB | 1:300 |
Arginase-1 (5% BSA blocking) | Cell Signaling (Rabbit, 9819S) | WB | 1:1000 |
BrdU | Santa Cruz (Mouse, 555,627) | ICC | 1:500 |
CD3 (1% BSA, 5% goat serum blocking) | Abcam (Rabbit, AB5690) | IHC | 1:200 |
CD4 | Cedarlane (Sheep, AF6439) | WB | 1:100 |
CD86 | Abcam (Rabbit, AB53004) | ICC | 1:60 |
CS56 | Sigma (Mouse, C8035) | IHC | 1:150 |
DAPI | Sigma (D9542) | ICC IHC | 1:10000 |
FOXP3 | Cedarlane (Rat, 14-5773-82) | WB IHC | 1:200 1:100 |
Iba1 | Wako (Rabbit, 016-20,001) | WB | 1:1000 |
Iba1 | Wako (Rabbit, 019-19,741) | ICC IHC | 1:500 1:500 |
IL-10 | Cedarlane (Mouse, MAB519) | WB | 1:250 |
IL-10 (1% BSA, 5% Goat serum blocking) | R&D Systems (Rat, MAB417) | IHC | 1:200 |
IL-1β | Serotec (Rabbit, AAR15G) | WB | 1:1000 |
GFAP | Chemicon (Mouse, MAB360) | ICC | 1:800 |
GFAP | Cell Signaling (Mouse, #3670S) | WB | 1:5000 |
GFAP | Chicken | IHC | 1:800 |
GAPDH | Santa Cruz (Rabbit, sc-25,778) | WB | 1:1000 |
LAR | Santa Cruz (Rabbit, sc-25,434) | ICC | 1:50 |
LAR | BD (Mouse 610,351) | WB | 1:250 |
Mannose receptor | Abcam (Rabbit, ab64693) | ICC | 1:400 |
Olig2 | Chemicon (Rabbit, AB9610) | ICC | 1:2000 |
OX42 (CD11b) | Serotec (Mouse, MCA275G) | IHC | 1:100 |
PTPσ | R&D (Goat, AF3430) | ICC | 1:50 |
TNFα | Serotec (Rabbit, AAR33) | WB | 1:1000 |