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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Inhibition of peripheral macrophages by nicotinic acetylcholine receptor agonists suppresses spinal microglial activation and neuropathic pain in mice with peripheral nerve injury

Fig. 1

Accumulation of macrophages in the injured SCN after PSL. Mice were subject to partial sciatic nerve ligation (PSL) or sham surgery, and the sciatic nerve (SCN) was collected. Isolated cells from the SCN were analyzed by flow cytometry. Representative forward scatter (FSC-A; cell-surface area or size) versus side scatter (SSC-A; cell granularity or complexity) plots presenting total events collected from contralateral (a, undamaged) and ipsilateral (b, injured) SCN on day 1 after PSL are presented. Leukocyte gates used for subsequent analysis are shown (a, b). Representative FSC-A versus CD11b expression plot from leukocyte gate indicate the population of CD11b+ cells from total leukocytes in contralateral (a) and ipsilateral (b) SCN. c, e Quantitative analysis for the percentage of CD11b+ cells (c) and F4/80+ cells (e) in total events from intact (pre-injury), contralateral and ipsilateral SCN (PSL and sham) indicates the accumulation of CD11b+ leukocytes in the injured SCN on days 1 to 14. d Representative plot of F4/80 versus CD11b expression from leukocyte gate (b, right panels) in the ipsilateral SCN presents F4/80+ CD11b+ macrophages in the injured SCN on day 1 after PSL. f The percentage of F4/80+ macrophages in CD11b+ leukocytes are shown on days 1 to 14 after PSL. Data are presented as the mean ± SEM; n = 5–6. ***P < 0.001, **P < 0.01, *P < 0.05 versus pre-injury

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Journal of Neuroinflammation

ISSN: 1742-2094