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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Inhibition of peripheral macrophages by nicotinic acetylcholine receptor agonists suppresses spinal microglial activation and neuropathic pain in mice with peripheral nerve injury

Fig. 5

Suppression of microglial activation in the SDH after PSL by peripheral TC-2559. Mice were subject to PSL or sham surgery. TC-2559 was administered (p.n., 20 nmol) on days 0, 1, 2, and 3, and the lumbar spinal cord (L4–5) was collected. Expression of Iba1 and CD68 in the spinal dorsal horn (SDH) on day 7 after PSL was analyzed by immunohistochemistry. a Representative micrographs of Iba1 in the contralateral side and Iba1, CD68 and merged images in the ipsilateral sides of the SDH are presented as lower magnification. Scale bars = 100 μm. b Representative micrographs of Iba1, CD68 and merged images are presented as higher magnification from the square in the ipsilateral SDH (a; lateral area). Scale bars = 20 μm. Quantitative analyses of Iba1 fluorescence intensity (c) and the number of Iba1+ microglia (d) within the square of 100 × 100 μm2 (a; medial area) in the contralateral and ipsilateral SDH on day 7 after PSL are shown. Data are presented as the mean ± SEM; n = 5. **P < 0.01 versus sham. #P < 0.05 versus PSL/Veh

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Journal of Neuroinflammation

ISSN: 1742-2094