Fig. 5

H19 stimulates p-Stat3 and c-Myc expression. a, b Protein levels of p-Stat3 and c-Myc in the hippocampal tissue samples from patients with TLE (n = 4) (a) and the hippocampus of epileptic rats 7 days after KA injection (n = 3) (b), as determined by western blotting. c, d Quantification of p-Stat3 and c-Myc protein levels in the CA3 subfield of the hippocampus of H19 overexpression (c) and H19 knockdown (d) rats with or without KA treatment for 7 days (n = 3–4). Protein bands were quantified by densitometry and normalized to the level of GAPDH. Data represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. NC rats injected with empty AAV vectors, Scr rats injected with scrambled AAV vectors, ShRNA rats injected with AAV vectors containing short hairpin RNA targeting H19