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Table 1 Primer sequences for RT-PCR analysis

From: Galantamine improves cognition, hippocampal inflammation, and synaptic plasticity impairments induced by lipopolysaccharide in mice

Target mRNA sequences Primer sequence Annealing Tm (°C)
GFAP 5′-AAGCAGATGAAGCCACCCTG-3′
5′-GTCTGCACG-GGAATGGTGAT-3′
59
CD11b 5′-CAGATCAACAATGTGACCGTATGGG-3′ 5′-CATCATGTCCTTGTACTGCCGCTTG-3′ 66
IL-1β 5′-TTGACGGACCCCAAAAGATG-3′
5′-AGAAGGTGCTCATGTCCTCA-3′
59
IL-6 5′-CGGAGAGGAGACTTCACAGAG-3′
5′-CATTTCCACGATTTCCCAGA-3′
59
TNF-α 5′-TATGGCTCAGGGTCCAACTC-3′
5′-GGAAAGCCCATTTGAGTCCT-3′
59
β-actin 5′-ATGGTCACGCACGATTTCCC-3′
5′-GAGACCTTCAACACCCCAGC-3′
59
IL-6 5′-AGACTTCCATCCAGTTGCCTTCTTG-3′
5′-CATGTGTAATTAAGCCTCCGACTTGTG-3′
59
IL-1β 5′-TTCAGGCAGGCAGTATCACTCATTG-3′
5′-ACACCAGCAGGTTATCATCATCATCC-3′
59
TNF-α 5′-GCGACGTGGAACTGGCAGAAG-3′
5′-GAATGAGAAGAGGCTGAGACATAGGC-3′
59
GAPDH 5′-GGTGAAGGTCGGTGTGAACG-3′
5′-CTCGCTCCTGGAAGATGGTG-3′
60