Fig. 5
From: β-arrestin2 functions as a key regulator in the sympathetic-triggered immunodepression after stroke
Knocking down ARRB2 expression by siRNA. siRNA targeting ARRB2 (LV3-β-arrestin2, 5′-GGACACCAACCTCATTGAATT-3′) and its empty vector (LV3-NC, 5′-TTCTCCGAACGTGTCACGT-3′) were transfected into THP-1 monocytes. After that, cells were subsequently differentiated into macrophages with 1.28 μM of PMA (5 × 105 cells/well) for 48 h. The transfection efficiency of each group was detected by a fluorescence microscope (×100). a–b The white-light photographs of monocyte-derived macrophages which are transfected with the ARRB2 downregulation siRNA (LV3-ARRB2) and the negative control siRNA (LV3-NC). c–d The fluorescence photographs of monocyte-derived macrophages which are stably transfected with LV3-ARRB2 and LV3-NC