Fig. 4

Langerhans cells associate intimately with epidermal substance P (SP) and calcitonin gene-related peptide (CGRP) immunostained sensory axons in the hind paw skin collected 3 weeks after tibia fracture. Top panels are double immunohistochemical staining of SP (red) and CD-207 (Langerin, a Langerhans cell marker, green) in a dorsal hind paw skin section, bottom panels are immunostaining of CGRP (red) and langerin (green) in a dorsal hind paw skin section. These representative photomicrographs demonstrate the close approximation of the SP and CGRP containing sensory afferent neurons and the Langerhans cells in the epidermis. Scale bar = 20 μmLangerhans cells associate intimately with epidermal substance P (SP) and calcitonin gene-related peptide (CGRP) immunostained sensory axons in the hind paw skin collected 3 weeks after tibia fracture. a Double immunohistochemical staining of SP (red) and CD-207 (Langerin, a Langerhans cell marker, green) in a dorsal hind paw skin section. b Immunostaining of CGRP (red) and langerin (green) in a dorsal hind paw skin section. These representative photomicrographs demonstrate the close approximation of the SP and CGRP containing sensory afferent neurons and the Langerhans cells in the epidermis. Scale bar = 20 μm