Fig. 7

Src inhibitor PP2 attenuated IL-1β-mediated suppression of cLTP-induced synaptic plasticity. a Gel image shows Western blots of phosphorylated Src (P-Src). Hippocampal neurons were exposed to IL-1β for 20 min. b Quantification of the blots shown in a. Data are mean ± SEM from three independent experiments expressed in terms of P-Src obtained in the control cultures (*p < 0.05, ANOVA). c Representative fluorescence images show F-actin labeled by phallodin in unstimulated control cells and cells treated with cLTP in the presence or absence of IL-1β with or without PP2 (10 μM). Scale bars, 5 μm. d Quantification of the images shown in c. Data are mean ± SEM from three independent experiments expressed in terms of control (*p < 0.05, ANOVA). e Representative fluorescence images show the expression of sGluA1 in unstimulated control cells and cells treated with cLTP in the presence or absence of IL-1β with or without PP2. Scale bar, 10 μm. f Quantification of the images shown in e. Data are mean ± SEM from three independent experiments expressed in terms of control (*p < 0.05, ANOVA)