Fig. 7
TNF blockade reduces EDNRB expression during progressive EAE in GFAPγR1Δ mice. a Confocal images (Z-stack) of spinal cords stained for EDNRB (green), DAPI (blue), and GFAP (astrocytes, red) or CD11b (macrophages/microglia, red) in WT, GFAPγR1Δ and GFAPγR1Δ + αTNF at d30 p.i. Images are representative of six to eight separate fields from two individual mice per group and two individual experiments. Scale bar, 50 μm. b Graph showing EDNRB positive area (mm2) per field at d19 and d30 p.i. Data represent mean ± SEM. P values determined by Wilcoxon rank-sum test. GFAPγR1Δ in all panels represents GFAPγR1Δ mice treated with isotype control mAb