Fig. 2
From: Lewy body-like alpha-synuclein inclusions trigger reactive microgliosis prior to nigral degeneration
α-Syn inclusions in the SNc exhibit oligomeric and fibrillary conformations and Lewy body-like characteristics. a–c Representative images of Lewy-body-like intraneuronal pSyn inclusions in the substantia nigra pars compacta (SNc) at 1 month p.i. show pathology is localized to the ipsilateral SNc. d–f Adjacent SN tissue sections stained for O2 (oligomeric/fibrillar α-syn conformation specific) and F2 (g–i) (fibrillar-specific conformation) α-syn reveal that many intraneuronal inclusions possess mature, fibrillar inclusions of α-syn. j Percent of pSyn inclusions with either oligomeric/fibrillar (O2) or predominantly fibrillar (F2) immunoreactivity and estimated proportion of pSyn inclusions that are oligomeric only. Data represent mean ± SEM. Scale bars (b, e, h) = 50 μm, (c, f, i) = 10 μm. k Endogenous α-syn immunoreactivity in the SNc and SNr. l Adjacent tissue sections exposed to proteinase-K reveal the absence of soluble α-syn in the SNr and the presence of insoluble, neuronal inclusions of α-syn in the SNc. m Thioflavin-S fluorescence of amyloid structure present in SNc neurons. Scale bars (k, l, m) = 50 μm, (insets) = 25 μm. Abbreviations: α-syn = alpha-synuclein; p.i. = postinjection; pSyn = α-syn phosphorylated at serine 129; O2 = oligomeric/fibrillar α-syn antibody; F2 = fibrillar α-syn only antibody; SNc = substantia nigra pars compacta; SNr = substantia nigra pars reticulata; SEM = standard error of the mean