Fig. 5
From: Cannabinoid CB2 receptors in the mouse brain: relevance for Alzheimer’s disease
EGFP expression is limited to plaque-associated microglial cells but is absent in neurons and astrocytes in CB2EGFP/f/f/5xFAD mice. a–c Z-stack showing that EGFP expression (a) was evident in microglial cells (b) located in close association to amyloid-enriched neuritic plaques, as revealed by methoxy-XO4 (c). However, microglial cells not linked with these pathological structures (arrow in b) showed reduced EGFP signal. d Orthogonal view in Z axis of the cluster of microglial EGFP+ cells shown in (a–c). Note the intimate contact established by microglial processes into the neuritic plaque. e–l Neurons (NeuN+ cells; e–h) nor astrocytes (GFAP+ cells; i–l) showed no EGFP signal. Scale bars, 25 μm