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Table 1 Real-time PCR primers (F, forward primer; R, reverse primer) and size of amplicon

From: Urate inhibits microglia activation to protect neurons in an LPS-induced model of Parkinson’s disease

Primer

Forward (5′-3′)

Reverse (5′-3′)

Mouse IL-1β

CTgTgTCTTTCCCgTggACC

CAgCTCATATgggTCCgACA

Mouse TNF-α

CAGCCGATGGGTTGTACCTT

TGTGGGTGAGGAGCACGTAGT

Mouse β-actin

TgCTgTCCCTgTATgCCTCT

TTgATgTCACgCACgATTTC

Rat IL-1β

TCTGTGACTCGTGGGATGAT

GGAGAATACCACTTGTTGGC

Rat TNF-α

ACTCCCAGGTTCTCTTCAAG

CAGAGAGGAGGCTGACTTTC

Rat GAPDH

TGACATCAAGAAGGTGGTGAAGC

GGAAGAATGGGAGTTGCTGTTG

  1. TNF-α, IL-1β, β-actin, and GAPDH genes were amplified using the forward and reverse primers listed in the above
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Journal of Neuroinflammation

ISSN: 1742-2094