Fig. 7

PCNA expression in immune cells and Müller glia at 72 h post-ouabain injection. Representative images of retinal cryosections at 72 h post-ouabain injection (72 hpi) stained for L-plastin to mark immune cells (green), glutamine synthetase to mark Müller glia (GS, red), PCNA (white), and DAPI (blue). A L-plastin and DAPI. B GS and DAPI. C L-plastin, GS, and DAPI. A’ L-plastin, PCNA, and DAPI. B’ GS, PCNA, and DAPI. C’ Four color merge of all stains. Nearly all PCNA+ nuclei can be attributed to immune cells or Müller glia at this time point. Asterisk (*) in A’ and C’ denotes L-plastin+, PCNA+ cell. Yellow arrows in B’ and C’ are provided to emphasize selected GS+ and PCNA+ cells. Occasionally, we observed PCNA+ nuclei at the vitreal surface at 3 dpi that could not be attributed to L-plastin+ or GS+ cells (C’, pink arrow). Scale bar in A (applies to all images) = 20 μm