Fig. 3

NADPH oxidase deletion attenuated the upregulation of proconvulsive cytokines in mouse brain following LPS stimulation. Wild-type (WT), gp91^phox−/− and P47^phox−/− mice were treated with saline (vehicle) or 4 mg/kg LPS (i.p.). Mouse brains (n = 5 per genotype) were harvested 1.5 h after LPS injection. The mRNA levels of TNFα, IL-1β, CCL2, IL-6, IL-10, and IL-12 was determined by RT-qPCR (a-f). Cytokine mRNA expression was normalized to β-actin mRNA expression (internal control). Fold change was calculated by comparing the expression of each cytokine of the LPS-treated mice to that of the genotype-matched saline-treated mice. Data present the mean ± SEM. Bonferroni post hoc test vs. corresponding WT control; *p < 0.05, **p < 0.01, ***p < 0.001