Fig. 3

Attenuated virus-specific CD8 T cell expansion and function in SCI mice after virus infection. Mice received SCI and 7 days later were infected with X31 i.n. After 7 days, lungs were collected and CD8 T cells were analyzed. Representative dot plots of a NP and b PA tetramer staining. Cells were gated for CD8 T cells. The absolute number of a NP and b PA tetramer-positive CD8 T cells in the lung were determined. Cells were cultured ex vivo with NP or PA peptides. After 4 h, CD8 T cells were stained for intracellular IFNγ and granzyme B production. The absolute number of IFNγ-positive cells was determined after c PA and d NP stimulation. e, f MFI of granzyme B expression in IFNγ⁻ and IFNγ⁺ CD8 T cells. *p < 0.05 by one-way ANOVA. Data represent two independent experiments with at least four mice per group per experiment