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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: RNA sequencing analysis reveals quiescent microglia isolation methods from postnatal mouse brains and limitations of BV2 cells

Fig. 6

Immune responses of primary microglia and BV2 cells to LPS. Primary microglia isolated by the CD11b MACS method and BV2 cells were treated with 100 ng/ml LPS for 24 h. a Representative gene expression in primary microglia and BV2 cells measured by quantitative RT-PCR. Expression level was normalized to respective vehicle control. b Heat map and hierarchical clustering of detectable 34 proteins released from primary microglia and BV2 cells measured by Luminex Multiplex. The minimal and maximal secretion level of each protein was indicated. Data are shown as mean + SD of triplicates. Experiment repeated twice independently. Unpaired t test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

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