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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: RNA sequencing analysis reveals quiescent microglia isolation methods from postnatal mouse brains and limitations of BV2 cells

Fig. 7

TGFβ signaling in primary microglia and BV2 cells. a Transcriptional expression of TGFβ signaling-related genes in primary microglia and BV2 cells by RNA-Seq. Data are shown as mean + SD, n = 4. b Transcriptional expression of TGFβ signaling-related genes in primary microglia and BV2 cells by quantitative RT-PCR. Data were normalized to the expression level in BV2 cells. Data are shown as mean + SD of triplicates. Experiment repeated twice independently. c Western blots were probed with the antibodies against p-Smad2, Smad2, and GAPDH. d Quantification of p-Smad2 signal intensity normalized to Smad2. Data are shown as mean + SD. Unpaired t test. ***P < 0.001; ****P < 0.0001

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