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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: RNA sequencing analysis reveals quiescent microglia isolation methods from postnatal mouse brains and limitations of BV2 cells

Fig. 8

Chemotaxis comparison between primary microglia and BV2 cells. a Transcriptional expression of C5a, C5ar1, and C5ar2 in primary microglia and BV2 cells by RNA-Seq. Inserted scheme depicting the expression of C5a and C5ar2. Data are shown as mean + SD, n = 4. Unpaired t test, ****P < 0.0001. b Transcriptional expression of C5ar1 and C5ar2 in primary microglia and BV2 cells by quantitative RT-PCR. Data are shown as mean + SD of triplicates. Unpaired t test, ****P < 0.0001. c Time-dependent chemotaxis of primary microglia and BV2 cells upon 11 nM C5a monitored by IncuCyte. Chemotactic index was obtained by normalization to random migration. Data are shown as mean + SD, n = 4. Two-way ANOVA, P(time) < 0.0001; P(cell type) < 0.0001; P(interaction) < 0.0001

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