Fig. 2
Screening of novel TNF-R1 inhibitors showed modulated post-traumatic sleep. Compounds were screened at two doses (high dose 20 mg/kg; low dose 2 mg/kg) for efficacy in modulating post-traumatic sleep compared to vehicle-treated and uninjured sham mice. Compounds were administered immediately following injury or sham procedure. A repeated measure two-way ANOVA was used to analyze main effect of treatment on percent sleep followed by Sidak’s multiple comparisons test when appropriate. Cumulative minutes slept was analyzed between sham and vehicle-treated using an un-paired t test, and across treatment groups using a one-way ANOVA followed by Tukey’s multiple comparisons test where appropriate. a, b Vehicle-treated brain-injured mice had a significant main effect of treatment on percent sleep (F1,11 = 6.835, p = 0.0241) and cumulative minutes spent sleeping (t11 = 2.614, p = 0.0241) compared to uninjured shams. c, d While both high- and low-dose compound C7 modulated post-traumatic sleep, there was no significant treatment effect between brain-injured mice treated with C7 compared to vehicle on percent sleep (F2,9 = 3.135, p = 0.0926) but there was an overall effect on cumulative minutes spent sleeping (F3,15 = 3.917, p = 0.0300). e, f There was an overall significant treatment effect between brain-injured mice treated with SGT compared to vehicle on percent sleep (F2,10 = 5.274, p = 0.0273) and cumulative minutes spent sleeping (F3,16 = 5.641, p = 0.0078). g, h Compound F002 did not significantly modulate percent sleep (F2,10 = 0.2786, p = 0.7625) or cumulative minutes spent sleeping (F3,16 = 2.252, p = 0.1217). Sleep groups; sham n = 7, vehicle-treated n = 6, C7-low n = 2, C7-high n = 4, SGT-low n = 4, SGT-high n = 3, F002-low n = 4, F002-low n = 3