Fig. 1

Treatment of rat cortical microglia with Apo-SAA or LPS upregulates in a time-dependent manner mRNA for NLRP3. Cultures were treated the day after plating with 0.5 μg/ml recombinant human Apo-SAA or 0.1 μg/ml LPS and processed 1, 3, 6, and 24 h later for q-PCR, as detailed in the “Methods” section. Data are presented as relative expression level (normalized with respect to β-actin (βACT)) at each time point and are mean ± sem, n = 6. Control values (which were at the limit of detection) were omitted for clarity. Apo-SAA (■); LPS (▓). For LPS, *p < 0.05 and **p < 0.01 vs 1 h; for Apo-SAA, °°p < 0.01 and °°°p < 0.001 vs 1 h