Fig. 4From: Targeted intracerebral delivery of the anti-inflammatory cytokine IL13 promotes alternative activation of both microglia and macrophages after strokeLocalization and cell density of microglia and macrophages 2Ā weeks after ischemic stroke. a Representative coronal T2-weighted MR images are displayed above the corresponding immunohistochemistry images of all experimental groups. The hyperintense regions in the coronal MR images show the ischemic lesion 2Ā days post stroke. bāc Fluorescent microscopic images of brain coronal sections of all experimental groups represent the distribution of CX3CR1eGFP microglia and CCR2RFP macrophages at day 14 after stroke; b localization of eGFP+ microglia and RFP+ macrophages in ipsilateral cortical and striatal ischemic lesions, and c in contralateral cortical and striatal intact regions. Immunofluorescence colors: blue, TOPRO; green, microglia; red, macrophages. dāf Representative stacked column graphs show the cell density quantification of all experimental groups at day 14 after stroke. d Evaluation of microglia/macrophage cell density in the whole hemisphere. No significant difference was observed in the total number of eGFP+ microglia and RFP+ macrophages between the three experimental groups. CX3CR1eGFP/+ cell density is significantly higher than CCR2RFP/+ cell density in all three experimental groups at day 14 after stroke. In intact contralateral hemisphere, eGFP+ microglia was the main population. e Further evaluation of microglia and macrophage cell density in cortical lesion areas, and f in striatal lesion areas. The cell density of recruited microglia and macrophages was higher in large cortical lesion areas compared to striatal lesion areas. Data represent meanāĀ±āSD. The data were compared between the three experimental groups using a parametric one-way ANOVA test with Bonferroniās post hoc test. To compare the CX3CR1eGFP/+ cell density to CCR2RFP/+ cell density in each group, an independent one-tailed Studentās t test was usedBack to article page