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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Targeted intracerebral delivery of the anti-inflammatory cytokine IL13 promotes alternative activation of both microglia and macrophages after stroke

Fig. 7

Transplantation of IL13-producing MSCs increases the number of recruited neuroprotective microglia and macrophages at day 14 after ischemic stroke. a Representative fluorescent microscopic images of ischemic cortical region show the expression of Arg-1 on CX3CR1eGFP/+ and CCR2RFP/+ cells in all three experimental groups at day 14 after stroke. Illustrative 20× close-up magnification display the changes in Arg-1 expression by microglia and macrophages. Immunofluorescence colors: blue, TOPRO; green, microglia; red, macrophages; white, Arg-1. b Detailed phenotypic quantitative analysis of CX3CR1eGFP/+ and CCR2RFP/+ cells expressing Arg-1 showed a significant increase in the number of GFP+RFPArg-1+ and GFPRFP+Arg-1+ cells in IL13-MSC-treated group, in comparison to both control groups. c The corresponding exploded pie charts show the distribution of Arg-1 expression by GFP+ and RFP+ cells in all three experimental groups. In the pie chart, GFP+ microglia and RFP+ macrophages are encircled in green and red, respectively. In the IL13-MSC group, the Arg-1+ fraction of macrophages is significantly higher than that of microglia. n = 5–6 mice in each group. Data represent Mean ± SD. The data were compared between the three experimental groups using a parametric one-way ANOVA test with Bonferroni’s post hoc test

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