Table 2 List of primary and secondary antibodies used for immunofluorescence staining
Antibody | Host | Company | Catalog ID | Dilution | Incubation conditions |
|---|---|---|---|---|---|
IRAK1 | Rabbit | Abcam | ab238 | 1:200 | Overnight 4 °C |
TRAF6 | Rabbit | Abcam | ab181622 | 1:200 | Overnight 4 °C |
pNF-kB (p65) | Rabbit | Abcam | ab86299 | 1:200 | Overnight 4 °C |
CGRP | Goat | LifeSpan BioSciences | LS-C122785 | 1:500 | Overnight 4 °C |
PGP9.5 | Guinea pig | Abcam | ab10410 | 1:100 | Overnight 4 °C |
Anti-rabbit IgG Alexa Fluor 488 | Donkey | Jackson ImmunoRresearch | 711-545-152 | 1:400 | 1Â h RT |
Anti-goat IgG Alexa Fluor 594 | Donkey | Jackson ImmunoRresearch | 705-585-147 | 1:400 | 1Â h RT |