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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Complement components are upregulated and correlate with disease progression in the TDP-43Q331K mouse model of amyotrophic lateral sclerosis

Fig. 3

Expression and localisation of C1q in the spinal cord of non-transgenic, TDP-43WT and TDP-43Q331K mice at 16 months of age. a The immunoreactive area of C1q in the spinal cord of non-transgenic (NTg, orange bars), TDP-43WT (green bars) and TDP-43Q331K (blue bars) at 16 months. Data are expressed as means ± SEM (n = 4 mice/group, *P < 0.05, one-way ANOVA with Tukey’s post hoc test). bj Double immunolabelling of C1q (red) with cellular markers (green) for motor neurons (ChAT; bd), microglia (Iba-1; eg) and astrocyte (GFAP; hj) in the ventral lumbar spinal cord of NTg, TDP-43WT and TDP-43Q331K mice at 16 months of age. C1q was co-localised with ChAT-positive motor neurons (d, white arrow) and microglia (g, white arrow) in TDP-43Q331K mice. Note that white arrows indicate red and green fluorescent signal merge to orange. In TDP-43Q331K mice, immunolabelling of C1q was also evident on other cell types, indicated by lack of co-localisation with anti-ChAT, anti-Iba-1 and anti-GFAP (d, g, j). Scale bars for all panels = 20 μm

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