Fig. 1

Microglial DICER protein is specifically downregulated in the MPTP model. a, b Upper—representative immunoblots of total lysates from the primary cultured microglia incubated with the indicated concentrations of MPP⁺ for 12 h and probed with the antibodies for DICER (a), Ago2, and Drosha (b). Lower—statistics (n = 4 for DICER, n = 5 for Ago2, and n = 4 for Drosha). c Upper—representative immunoblots of DICER from mouse neurons or astrocytes treated with the indicated concentrations of MPP⁺ for 12 h. Lower—statistics (n = 4 for neurons, n = 6 for astrocytes). d Upper—representative immunoblots of total lysates from the BV2 cells incubated with the indicated concentrations of MPP⁺ for 12 h and probed with the antibodies for DICER, Ago2, and Drosha. Lower—statistics (n = 5 for DICER and n = 4 for Ago2 and Drosha). e Left—representative immunoblots of total lysates from the CD11b+/CD45low-labeled microglia sorted from mouse ventral mesencephalon (VM) by flow cytometric 3 days after the administration of saline or MPTP and probed with the antibodies for DICER, Ago2, and Drosha. Right—statistics (n = 4 for DICER, Ago2, and Drosha). f Upper—representative immunoblots of total lysates from the GLAST+ labeled astrocytes sorted from VM by flow cytometric 3 days after administration of saline or MPTP and probed with the DICER antibody. Lower—statistics (n = 4 for DICER). Data are mean + SEM. *p < 0.05, **p < 0.01. Unless stated, α-tubulin (α-Tub) or Actin as loading controls. kDa, kilodalton