Fig. 3

Inhibition of JNK suppresses microglial DICER degradation induced by MPP⁺. a–f Representative immunoblots of total lysates from microglia (a–c) and BV2 cells (d–f) exposed to MPP⁺ for the indicated times and probed with the antibodies for p-ERK and ERK (a, d), for p-JNK and JNK (b, e), and for p-p38 and p38 (c, f). g–j Representative immunoblots of total lysates from microglia (g–i) and BV2 cells (j) treated with MPP⁺ or/and U0126 (g, j,10 μM), SP600125 (SP, 10 μM) (h, j), and SB203580 (SB, 10 μM) (i, j) using the antibodies against DICER. Lower—statistics (g–i; n = 3 for g, n = 4 for h, and n = 3 for i, and n = 3 for j). k Upper—representative immunoblots of immunoprecipitates from total lysates of BV2 cells pretreated SP600125 for 2 h and then incubated with MG115 with/without MPP⁺ and immunoblotted with anti-ubiquitin antibody. Lower—immunoblots of the DICER precipitates. Data are mean + SEM. *p < 0.05