Table 1 Antibodies used for flow cytometric analysis of cultured bone marrow-derived macrophages and for sorting macrophages from injured sciatic nerve
From: Temporal changes in macrophage phenotype after peripheral nerve injury
Antibody/probe | Clone | Fluorophore | Distributor/Cat No. | Specificity |
|---|---|---|---|---|
CD16/32 | 2.4G2 | BV-605 | BD Biosciences/563006 | Pan macrophage |
F4/80 | BM8 | PE-Cy7 | eBioscience/25-4801 | Pan macrophage |
CD11b | M1/70 | Pacific Blue | BioLegend/101224 | Pan macrophage |
CD14 | Sa2-8 | PE | eBioscience/12-0141 | Pan macrophage |
Arg1 | Â | PE | R&D Systems/IC5868P | M2 |
Nos2 | CXNFT | APC | eBioscience/17-5920 | M1 |
Thy1.2 | 53-2.1 | APC | eBioscience/17-0902 | Fibroblast |
CD19 | ID3 | APC | eBioscience/17-0193 | Pan B cell |
Ly6G | 1A8-Ly6g | APC | eBioscience/17-9668 | Granulocyte |
Siglec F | ES22-10D8 | APC | Miltenyi Biotec/130-102-241 | Eosinophil |
Ter119 | Ter119 | APC | eBioscience/17-5921 | RBC |
CD31 | MEC 13.3 | APC | BD Biosciences/551262 | Endothelial cell |
CD3e | 17A2 | APC | eBioscience/17-0032 | T cell |
Propidium iodide | Â | na | eBioscience/00-6990 | Dead cell/DNA |
Zombie Aqua | Â | na | BioLegend/423101 | Fixable viability marker |