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Table 2 Longitudinal chemokine levels in all fractions of BBB models

From: Longitudinal chemokine profile expression in a blood-brain barrier model from Alzheimer transgenic versus wild-type mice

Chemokines

BBB fractions

3M

6M

12M

WT

AD

WT

AD

WT

AD

CX3CL1

M1

83.46 ± 20.27

74.53 ± 38.46

139.50 ± 31.67

99.73 ± 23.25

99.76 ± 17.93

70.38 ± 10.29

Abluminal lysates

0.98 ± 0.54

1.175 ± 0.45

1.13 ± 0.42

1.01 ± 0.45

0.79 ± 0.27

1.03 ± 0.19

CCL2

M1

20,371 ± 9295

13,182 ± 4167

9458 ± 2368

8076 ± 2567

12,963 ± 3943

4383 ± 1520

PB lysates

1039 ± 307

577.60 ± 284.30

221.40 ± 59.11

264.10 ± 102.50

1007 ± 547.80

120.70 ± 31.74

Luminal lysates

531.10 ± 227.70

335.10 ± 126.20

214.50 ± 54.57

480.30 ± 226.80

270 ± 76.21

114.40 ± 25.77

Abluminal lysates

393.60 ± 142

278.50 ± 172.10

139 ± 40.94

269.20 ± 126.40

249.90 ± 137.9

53.42 ± 10.36

CCL3

M2

79.96 ± 25.51

33.02 ± 6.81

311.50 ± 126.40

231.70 ± 86.12

717.40 ± 271.30

179.90 ± 110

PB lysates

2.33 ± 2.18

7.93 ± 1.89

5.24 ± 1.78

2.77 ± 1.34

14.19 ± 6.34

4.48 ± 2.03

Luminal lysates

14.55 ± 4.93

11.62 ± 2.59

14.68 ± 3.02

12.09 ± 3.10

28.71 ± 7.63

7.83 ± 2.30

Abluminal lysates

7.82 ± 3.43

0.31 ± 0.16

5.24 ± 1.31

2.89 ± 1.34

8.06 ± 2.67

3.13 ± 1.53

CCL4

M1

375 ± 125.90

333.50 ± 83.34

599.40 ± 186.90

675.50 ± 231

1135 ± 354.20

241.40 ± 110.30

M2

332 ± 117.40

167.80 ± 41.30

624.50 ± 254.20

880.90 ± 325.20

1503 ± 463.10

342.30 ± 226.90

PB lysates

6.98 ± 3.55

5.99 ± 2.22

11.99 ± 5.28

10.51 ± 3.60

18.95 ± 7.31

5.76 ± 3.91

Luminal lysates

11.15 ± 5.06

8.34 ± 3.39

11.76 ± 4.87

13.78 ± 4.89

15.95 ± 5.52

9.60 ± 3.35

Abluminal lysates

12.31 ± 3.28

2.46 ± 2.31

20.17 ± 7.61

13.98 ± 5.17

267.60 ± 117.20

4.40 ± 2.60

CCL5

M1

16.96 ± 7.98

14.84 ± 4.86

81.18 ± 40.05

62.77 ± 25.69

128.30 ± 63.82

13.07 ± 5.30

M2

15.02 ± 6.90

6.25 ± 3.13

82.91 ± 42.46

73.45 ± 38.29

138.60 ± 72.71

7.65 ± 1.99

PB lysates

0.81 ± 0.34

1.51 ± 0.32

2.98 ± 1.54

2.32 ± 0.81

7.77 ± 4.07

2.56 ± 1.58

Luminal lysates

2.43 ± 0.93

2.31 ± 0.43

6.58 ± 3.37

3.71 ± 1.35

5.46 ± 2.07

2.82 ± 1.00

Abluminal lysates

1.28 ± 0.62

0.34 ± 0.19

2.34 ± 1.06

1.55 ± 0.68

2.83 ± 1.40

1.19 ± 0.77

CXCL10

M1

526.30 ± 189.80

283.60 ± 81.98

304.50 ± 98.88

261.10 ± 74.48

318.40 ± 61.75

246.40 ± 79.64

M2

325.10 ± 109.50

189.30 ± 54.77

426.50 ± 203.50

505.10 ± 202

438.30 ± 177.6

185.10 ± 42.96

PB lysates

21.98 ± 11.41

20.06 ± 12.06

49.71 ± 24.08

46.80 ± 17.02

38 ± 20.53

60.77 ± 27.67

Luminal lysates

22.87 ± 14.16

18.91 ± 5.84

35.84 ± 13.50

46.72 ± 14.66

46.18 ± 17.28

17.19 ± 7.94

Abluminal lysates

13.54 ± 9.27

8.03 ± 4.23

20.75 ± 8.35

23.58 ± 9.54

28.95 ± 15.76

13.91 ± 9.18

  1. Chemokine levels (CX3CL1, CCL2, CCL3, CCL4, CCL5, and CXCL10) in all fractions of BBB models at 3 (WT n = 4–5, AD n = 5–6), 6 (WT n = 10–11, AD n = 8–9), and 12 months (WT n = 8–11, AD n = 4–6) were analyzed by the Luminex® xMAP® assay containing a mixture of beads specific for each chemokine as described in the “Methods” section. Chemokine levels are mean ± SEM and expressed in picograms per milliliter for luminal and abluminal media and in picograms per milligram of protein for cell lysates. Statistical analyses revealed no significant difference
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Journal of Neuroinflammation

ISSN: 1742-2094