Fig. 3

Macrophage accumulation is significantly diminished in the axotomized DRG of Ccr2^−/− mice compared to WT mice, while satellite glial cells and neutrophils are comparable between genotypes. Three and seven days after sciatic nerve transection, L4, L5 and L6 DRG were dissected and examined using flow cytometry. Both sham and axotomized nerves were analyzed. Gray boxes in a indicate CD11b⁺F4/80⁺ cells. In b, black boxes indicate CD11b⁺Ly6G⁻ cells and gray boxes indicate CD11b⁺Ly6G⁺ cells. Flow cytometric analysis of macrophage populations in the injured DRG (a, b) shows similar percentages of CD11b⁺F4/80⁺ and CD11b⁺Ly6G⁻ cells between genotypes at 3 days (c) and significant attenuation in Ccr2^−/− mice compared to WT mice at 7 days (d) post injury. A significant increase in the percentage of satellite glial cells after axotomy was seen in 3 days in Ccr2^−/− DRG alone, with a trend towards a significant increase over injured WT DRG (e). No differences were observed for CD11b⁺Ly6G⁺ neutrophils after injury in WT mice, while a trend towards a significant increase was observed 3 days after injury in Ccr2^−/− mice (f). Mean ± SEM, two-way ANOVA, Tukey’s post hoc test corrected (within individual time points). *p < 0.05. **p < 0.001. n = 3 mice per genotype per time point