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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Ex vivo model of epilepsy in organotypic slices—a new tool for drug screening

Fig. 3

Western blot analysis of αII-spectrin cleavage and Pro-caspase 3 of organotypic slices control (CTL) and with spontaneous epileptiform activity (EL). a, b Representative immunoblots of αII-spectrin (250 kDa), SBDP 145/150 (145/150 kDa), Pro-caspase 3 (32 kDa), and GAPDH (37 kDa). ce Densitometry analysis was performed with ImageJ software using GAPDH as internal control. c Full length αII-spectrin expression was similar between CTL and EL slices, while d the ratio SBDP 145/150 to αII-Spectrin was significantly upregulated in EL slices. e No differences were found in Pro-caspase 3 expression between CTL and EL slices. All values are mean ± SEM. n = 4, *p < 0.05, unpaired t test

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