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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Ex vivo model of epilepsy in organotypic slices—a new tool for drug screening

Fig. 7

Representative morphology of microglia in organotypic slices control (CTL) and with spontaneous epileptiform activity (EL) at 14 DIV. Images of Iba1-stained microglia (green) and Hoechst-stained nuclei (blue) were acquired on a confocal laser microscope (Zeiss LSM 710) with a 20x objective. Magnified images of the dashed areas are shown. a Most microglia in CTL slices have a ramified morphology, characteristic of a resting state. b EL slices depict microglia with a large cell body and retracted processes, corroborating an activated “bushy-like” state. c The diameter of microglia cell body was quantified with ImageJ software. In EL slices, microglia show a significant increase in cell body diameter in all hippocampal areas. All values are mean ± SEM. n = 50–70 microglia cells per hippocampal area per condition, from 3 independent cultures, ***p < 0.001, one-way ANOVA, followed by Bonferroni’s comparison test. Scale bars, 50 μm

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