Fig. 7
From: Ex vivo model of epilepsy in organotypic slices—a new tool for drug screening
Representative morphology of microglia in organotypic slices control (CTL) and with spontaneous epileptiform activity (EL) at 14 DIV. Images of Iba1-stained microglia (green) and Hoechst-stained nuclei (blue) were acquired on a confocal laser microscope (Zeiss LSM 710) with a 20x objective. Magnified images of the dashed areas are shown. a Most microglia in CTL slices have a ramified morphology, characteristic of a resting state. b EL slices depict microglia with a large cell body and retracted processes, corroborating an activated “bushy-like” state. c The diameter of microglia cell body was quantified with ImageJ software. In EL slices, microglia show a significant increase in cell body diameter in all hippocampal areas. All values are mean ± SEM. n = 50–70 microglia cells per hippocampal area per condition, from 3 independent cultures, ***p < 0.001, one-way ANOVA, followed by Bonferroni’s comparison test. Scale bars, 50 μm