Inflammation and blood-brain barrier breach remote from the primary injury following neurotrauma

Table 1 Summary of cohorts and numbers of animals per group

	Uninjured	Day 1	Day 3	Day 7	Day 14	Day 28
Cohort 1
- Microglia/macrophages in the optic nerve	n = 10*		n = 10	n = 10		n = 10
Cohort 2
- BBB analyses	n = 9–12 EB		n = 9–12 EB	n = 9–12 EB	n = 9–12 EB	n = 9–12 EB
- Microglia/macrophages in the optic tract	n = 9	n = 6 no EB	n = 6	n = 5–6	n = 4–5	n = 4
Cohort 3
- Cytokines	n = 8	n = 8	n = 8	n = 8

Day is the day after partial optic nerve transection injury. * For cohort 1, three groups of uninjured controls collected at days 3, 7, and 28 were compared and found to be not different (p ≤ 0.05); the 3-day controls were used for statistical comparisons. EB denotes intravenous injection of Evans blue for the specified animals. Numbers of animals per group are presented as a range if suitable sections were not available for specific brain regions. BBB analyses encompass assessments of Evans blue, caveolin, and fibrinogen immunoreactivity

ISSN: 1742-2094