Table 1 Method validation parameters for detection of 4-HHE in microglia cells
| Linear range (ng/mL) | Linearity | Matrix effect | Phree recovery | Sep-Pak recovery | Intraday | Interday | ||
|---|---|---|---|---|---|---|---|---|
| Accuracy (bias, %) | Precision (CV, %) | Accuracy (bias, %) | Precision (CV, %) | |||||
| 15.63–1000 | 0.9994 | 92.6% | 104.4% | 92.4% | 0.5% | 0.7% | 1.5% | 0.8% |