Table 2 Method validation parameters for detection of 4-HNE in microglia cells
Linear range (ng/mL) | Linearity | Matrix effect | Phree recovery | Sep-Pak recovery | Intraday | Interday | ||
|---|---|---|---|---|---|---|---|---|
Accuracy (bias, %) | Precision (CV, %) | Accuracy (bias, %) | Precision (CV, %) | |||||
6.25–250 | 0.9989 | 92.6% | 88.8% | 92.1% | 2.4% | 1.0% | 9.9% | 1.3% |