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Table 2 Method validation parameters for detection of 4-HNE in microglia cells

From: Unveiling anti-oxidative and anti-inflammatory effects of docosahexaenoic acid and its lipid peroxidation product on lipopolysaccharide-stimulated BV-2 microglial cells

Linear range (ng/mL) Linearity Matrix effect Phree recovery Sep-Pak recovery Intraday Interday
Accuracy (bias, %) Precision (CV, %) Accuracy (bias, %) Precision (CV, %)
6.25–250 0.9989 92.6% 88.8% 92.1% 2.4% 1.0% 9.9% 1.3%
  1. CV coefficient of variance