Fig. 5

Reduction of Nlrp3 decreased MPTP-induced NLRP3 inflammasome activation and TH neuronal loss in SNc from mice injected with LV3-siNlrp3 via the tail vein. a, b Representative blots and analysis of NLRP3 activation signaling makers in the midbrain samples from the LV3-NC or the LV3-siNlrp3 mice 7 days post MPTP treatment. c Representative images (left) and quantitative analyses (right) of the positive cell numbers showing immunochemical label microglia using the IBA-1 in the SNc of mice injected with the LV3-NC or the LV3-siNlrp3 7 days post MPTP treatment. d Representative images (left) and quantitative analyses (right) of positive cell numbers showing immunochemical label DA neurons using TH in the SNc of mice injected with the LV3-NC or the LV3-siNlrp3 7 days post MPTP treatment (n = 4 animals per condition). Samples were collected after 7 days of last MPTP injection. Data is presented as mean ± SEM, two-way ANOVA,*p < 0.05, **p < 0.01, ***p < 0.001 versus corresponding the saline group; ^#p < 0.05, ^##p < 0.01 versus the corresponding LV3-NC group