Fig. 8

a–i Validation of RNA Seq data using qPCR. Real-time qPCR validation of representative genes identified by RNA Seq data in the cortex and spleen that are affected by TBI and treatment with exosomes. All assays were run in triplicate, and data is expressed as the mean ± SEM. Data for each gene was analyzed by one-way ANOVA followed by Dunnet’s multiple comparison test. ^##p < 0.01 indicates that group was different from control. ANOVA for each graph are IL1ß cortex (F(3, 8) = 16,008); TNFα cortex (F(3, 8) = 5989); IFNG cortex (F(3, 8) = 1784); TrkC cortex (F(3, 8) = 814); IL10 cortex (F(3, 8) = 2980); RABL6 spleen (F(3, 8) = 4640); TNFα spleen (F(3, 8) = 286); E2F4 spleen (F(3, 8) = 516); PLAg2 spleen (F(3, 8) = 451)