Fig. 7

Effect of Bindarit and RS504393 on ethanol-induced neuronal death in the co-cultures of microglia/primary neurons. a Co-cultures of SIM-A9 cells and primary cortical neurons/cerebellar neurons were established described in the “Methods” section. The co-cultures were treated with MCP-1 (10 ng/ml in PBS) or EtOH (0.4%) for 48 h. The viability of neurons was determined by MTT as described in the “Methods” section. Data were the mean ± SEM of three independent experiments. *p < 0.05, statistically significant difference from primary neuronal culture alone. b SIM-A9 cells were treated with Bindarit (300 μM) or RS504393 (100 μM) for 12 h. After that, the inserts containing SIM-A9 cells were placed in the culture wells containing cortical neurons or cerebellar neurons. The co-cultures were then treated with EtOH (0.4%) for 48 h, and the viability of neurons was determined by MTT. Each data point was the mean ± SEM of three independent experiments. *p < 0.05, statistically significant difference from co-cultures without Bindarit or RS504393 treatment