Fig. 7
Activating GPR30 inhibited microglial Iba1 protein expression and the TLR4/ NF-κB pathway. a Western blotting analysis of Iba1 and TLR4 protein expression in microglia of the ischemic penumbra 24 h after reperfusion. The lower panel shows Iba1 and TLR4 and the corresponding β-actin bands. The histogram in the upper panel shows the results of the densitometric analysis. The data are expressed as the mean ± SD and analyzed by one-way ANOVA with Tukey’s post-test. ###p < 0.001 compared with the sham group. *p < 0.05, **p < 0.01, ***p < 0.001 compared with the vehicle group. n = 6 per group. b Western blotting analysis of the protein expression of Iba1 and TLR4 in primary microglia 12 h after the reintroduction of oxygen and glucose. The data are expressed as the mean ± SD and analyzed by one-way ANOVA with Tukey’s post-test. ##p < 0.01, ###p < 0.001 compared with the con group. *p < 0.05, **p < 0.01, ***p < 0.001 compared with the vehicle group. The data were pooled from six independent experiments. c TLR4 mRNA expression levels in primary microglia as detected by real-time PCR 12 h after the reintroduction of oxygen and glucose. The data are expressed as the mean ± SD and analyzed by one-way ANOVA with Tukey’s post-test. ###p < 0.001 compared with the con group. **p < 0.01, ***p < 0.001 compared with the vehicle group. The data were pooled from six independent experiments. d Western blotting analysis of TLR4 protein expression in LPS-activated primary microglia 12 h after G1 treatment. The data are expressed as the mean ± SD and analyzed by one-way ANOVA with Tukey’s post-test. ##p < 0.01 compared with the con group. *p < 0.05 compared with the vehicle group. The data were pooled from five independent experiments. e Cytoplasmic and nuclear levels of NF-kB in LPS-activated primary microglia 12 h after G1 treatment as detected by Western blot. The data are expressed as the mean ± SD and analyzed by one-way ANOVA with Tukey’s post-test. #p < 0.05, &&p < 0.01 compared with the indicated con group. *p < 0.05 compared with the vehicle group. The data were pooled from five independent experiments. LPS lipopolysaccharide